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In the morning on an empty stomach : in support of a slimming or well-being program

Once mixed, the final solution should be approximately isotonic if given IV (meaning it should contain appropriate solute concentration for safety)

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Keeping it moderate

Aliquots (500 L) of blood collected with ethylenediamine tetraacetic acid (EDTA) 5% were analyzed using an automatic hematologic analyzer ( Hematoclin 2.8 VET, Starlab, Salvador, BA, BRA) and blood samples (1000 L) collected without anticoagulant were used to obtain serum for analyses of oxidative stress (12 and 24 h post CLP) and cytokines (24 h post CLP)